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coimmunoprecipitation co ip assay hek293t cells (MedChemExpress)

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MedChemExpress coimmunoprecipitation co ip assay hek293t cells
Coimmunoprecipitation Co Ip Assay Hek293t Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek293t+cells/THAM/pm42412281-102-1-38
Average 97 stars, based on 215 article reviews

coimmunoprecipitation co ip assay hek293t cells - by Bioz Stars, 2026-09

97/100 stars

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Transfection:

Article Title: Palmitoylation of death receptor p75 NTR contributes to Alzheimer’s disease progression by regulating APP trafficking and degradation
Article Snippet: .. Briefly, transfected HEK293T cells or hippocampi from postnatal day 3 pups were lysed in 50 mM HEPES pH 7.2, 2% (w/v) SDS, 1 mM EDTA plus protease inhibitors (MCE). ..

Article Title: TGM2-mediated serotonylation of GPX4 confers ferroptosis resistance to promote gastric tumorigenesis
Article Snippet: .. The GPX4 (WT) and GPX4 (mutant) expression plasmids were transfected into HEK293T cells for 48 h, followed by treatment with CHX (MCE, HY-12320) at the indicated time points. ..

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation
Article Snippet: For co-immunoprecipitation between Creld1 and Na v channels, HEK293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher scientific, C11995500BT) supplemented with 10% (vol/vol) fetal bovine serum (ThermoFisher Scientific) and 100 U/ml penicillin-streptomycin (ThermoFisher Scientific, 15140122) in 15 cm dishes. .. HEK293T cells were transfected using polyethylenimine (PEI) (MCE, HY-K2014). ..

Article Title: Pathogenicity and Functional Analysis of Multi-Variant Allele of RPE 65 Causing Retinitis Pigmentosa
Article Snippet: Protein bands were visualized with a chemiluminescent detection reagent (WBKLS0500; MilliporeSigma) and quantified using ImageJ 1.52a (National Institutes of Health, Bethesda, MD). .. After 24 hours of transfection, HEK293T cells were treated with 100-μM cycloheximide (CHX, HY-N0901; MedChemExpress, Monmouth Junction, NJ) or CHX mixed with MG-132 proteasome inhibitor (HY-12320; MedChemExpress). ..

Mutagenesis:

Expressing:

Immunoprecipitation:

Article Title: The Rac1-USP11 feedback amplification loop: a radiation-activated engine driving radioresistance in hepatocellular carcinoma.
Article Snippet: Protein bands were quantified by densitometry (ImageJ), and Rac1 activity was normalized to total Rac1 and α-tubulin (loading control) levels. .. Huh7 and HEK293T cells were treated with 10 μM MG132 (MedChemExpress, USA) for 4 h. After treatment, the cells were washed with PBS and lysed for immunoprecipitation using 4 μg of anti-Rac1, anti-Myc, or antiFlag antibodies. ..